( C) The flow chart of data processing, 3D classification, and refinement. ( B) Representative cryo-electron microscopy (cryo-EM) image of the HsTMEM120A-CoASH complex in nanodiscs and 2D classification images.
The image of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the peak fraction is shown on the right. ( A) Size-exclusion chromatography of the HsTMEM120A-CoASH complex reconstituted in nanodiscs. One-way analysis of variance (ANOVA) with comparison to the vector. In panels ( C), (F), and (I), each bar represents mean ± sem, and the recorded cell number is labeled above the bar. ( I) Scatter plot of the maximal stretch-induced currents.
#RMSD IN COOT SOFTWARE PATCH#
( H) Current-pressure curves showing stretch-induced currents under inside-out patch configuration from P1-KO-HEK cells transfected with the indicated constructs in response to the increased negative pressures. ( G) Representative stretch-induced currents from P1-KO-HEK cells transfected with the indicated constructs under the inside-out patch configuration. ( F) Scatter plot of the maximal stretch-induced currents. The MmTMEM120A group includes data from both MmTMEM120A-mCherry- and MmTMEM120A-ires-GFP-transfected cells, while the HsTMEM120A group includes data from both HsTMEM120A-mCherry- and GFP- HsTMEM120A-transfected cells.
( E) Current-pressure curves showing stretch-induced currents under cell-attached patch configuration from P1-KO-HEK cells transfected with the indicated constructs in response to the increased negative pressures. ( D) Representative stretch-induced currents from P1-KO-HEK cells transfected with the indicated constructs under the cell-attached patch configuration. ( C) Scatter plot of the maximal poking-evoked whole-cell currents. The MmTMEM120A group includes data from both MmTMEM120A-mCherry- and MmTMEM120A-ires-GFP-transfected cells. ( B) Current-displacement curves showing poking-evoked whole-cell currents from P1-KO-HEK cells transfected with the indicated constructs in response to the increased probe displacement steps. ( A) Representative poking-evoked whole-cell currents from P1-KO-HEK cells transfected with the indicated constructs.
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